carbonic anhydrase II

CARBONIC ANHYDRASE II

Human, recombinant, folding state checked by NMR.
Full length, with mutation S2A
MW = 29.2 kDa
CAT # G07CA201

Packaging and prices - Technical Details - References

Packaging and Prices

Unlabeled Protein
Catalog n° Labeling Q.ty Price (€)
G07CA201 none 1 mg 550.00
G07CA201 none 5 mg 1,700.00
G07CA201 none 10 mg 2,770.00

Labeled Protein
Catalog n° Labeling Q.ty Price (€)
G07CA201n 15N 5 mg 1,850.00
G07CA201n 15N 10 mg 3,010.00
G07CA201cn 13C15N 5 mg 2,160.00
G07CA201cn 13C15N 10 mg 3,510.00


Special prices for bulk quantities available upon request.

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Description
MW = 29.1 kDa. Full length Carbonic Anhydrase II (Carbonate dehydratase II, Carbonic anhydrase C) cloned from human cDNA and expressed in E.coli. The protein consist of the human Carbonic Anhydrase II with a mutation Ser2Ala. (Swiss prot accession: P00918). Mass spectrometry (MALDI) showed that the first aminoacid (Met) is processed during the expression.

Sequence
        10         20         30         40         50
MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS 
        60         70         80         90        100
YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL 
       110        120        130        140        150
DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG 
       160        170        180        190        200
SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP 
       210        220        230        240        250
PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL 
       260
KNRQIKASFK   
Purity
> 95% by SDS-PAGE. The protein was observed as a single band migrating between 25 and 35 KDa.

Supplied as
2 mg/mL solution in Tris buffer 20mM pH 7.5, NaCl 150mM. The concentration is calculated from the absorbance at 280nm (ε280 = 54000 M-1cm-1).

Characteristics
Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated up to 1 mM.

Storage
-20°C. The protein is stable at 4°C for weeks and at 25°C for at least several days. .
Avoid repeated freeze/defrost cycles.

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References
Murakami, H., et al. Genomics. 1987 Oct;1(2):159-66.
Montgomery, J.C., Nucleic Acids Res. 1987 Jun 11;15(11):4687
Bertini, I., et al. J. Magn Reson B. 1994 Jul;104(3):230-9.

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