GB1

GB1 - Immunoglobulin G

Recombinant, from Streptococcus sp, folding state checked by NMR.
residues 303-357 with the mutation T303Q
MW = 6.2 kDa
CAT # G03GB101

Packaging and Prices - Technical Details - References

Packaging and Prices

Unlabeled Protein
Catalog n° Labeling Q.ty Price (€)
G03GB101 none 1 mg 100.00
G03GB101 none 5 mg 310.00
G03GB101 none 10 mg 500.00
G03GB101 none 20 mg 810.00

Labeled Protein
Catalog n° Labeling Q.ty Price (€)
G03GB101n 15N 5 mg 770.00
G03GB101n 15N 10 mg 1,385.00
G03GB101cn 13C15N 5 mg 1,230.00
G03GB101cn 13C15N 10 mg 2,155.00
G03GB101dcn 2H13C15N 5 mg 2,390.00
G03GB101dcn 2H13C15N 10 mg 3,380.00

Ready to use NMR standard
Catalog n° Labeling Tube Price (€)
G03GB101n_t3 15N 3 mm tube 540.00
G03GB101n_t5 15N 5 mm tube 770.00
G03GB101cn_t3 13C15N 3 mm tube 555.00
G03GB101cn_t5 13C15N 5 mm tube 1,075.00
G03GB101dcn_t3 2H13C15N 3 mm tube 1,780.00
G03GB101dcn_t5 2H13C15N 5 mm tube 2,655.00

Labeled microcrystals
Catalog n° Labeling Q.ty Price (€)
G03GB102cn 13C15N 5 mg 1,385.00
G03GB102cn 13C15N 10 mg 2,310.00
G03GB102dcn 2H13C15N 5 mg 2,750.00
G03GB102dcn 2H13C15N 10 mg 3,890.00

Special prices for bulk quantities available upon request.

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Description
MW = 6.2 kDa. GB1 (Immunoglobulin G-binding protein G, IgG-binding protein G) expressed in E.coli. The protein consists β-1 Immunoglobulin Binding Domain of Protein G, residues 303-357, with the mutation T303Q (T2Q). This mutation reduces the cleavage of the N-terminal Met residue (Swiss Prot accession P19909).

Sequence
       310        320        330        340        350
M-QYKLILNG KTLKGETTTE AVDAATAEKV FKQYANDNGV DGEWTYDDAT 
       
KTFTVTE
Purity
> 95% by SDS-PAGE. The protein was observed as a single band migrating at molecular weight <10 kDa.

Supplied as
1.0mg/ml in 20 mM Sodium Phosphate pH 7. The concentration is calculated from the absorbance at 280nm.(ε280=9970 M-1cm-1).

Characteristics
Under the above described conditions, to avoid precipitation or protein aggregation, the product can be concentrated up to 3mM.

Storage
-20°C. The protein is stable at 4°C for months at 25°C for at least two weeks.
Avoid repeated freeze/defrost cycles.
NMR USE: after the experiments return the sample at 4°C.

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References
H.L. Schmidt et al, J Phys Chem B. 2007 Dec 27;111(51):14362-9.
W.T. Franks, J Am Chem Soc. 2005 Sep 7;127(35):12291-305.

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