MMP19cat

MMP19 catalytic domain

Human, recombinant.
residues 101-256, swissprot accession Q99542
MW = 17600 Da.
EC # 3.4.24.-
CAT # G04MP19C

Packaging and prices - Technical Details - References

Packaging and Prices

Unlabeled Protein
Catalog n° Labeling Q.ty Price (€)
G04MP19C none 10 µg 200.00
G04MP19C none 5 x 10 µg 400.00

Special prices for bulk quantities, or labelled samples, available upon request.

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Description
MW = 17.6 kDa calculated. Matrix Metalloproteinase-19 isorform 1 RASI-1 (MMP-19, Matrix metalloproteinase RASI, MMP-18) catalytic domain cloned from human cDNA, expressed in E. coli. The enzyme consists of the catalytic domain of human MMP-19, (residues 101-256 - Swiss prot accession Q99542) with an additional methionine at N-term.

Purity
> 95% by SDS-PAGE. The protein was observed as a single band migrating at a molecular weight of between 19.5 and 14.4 kDa

Specific activity
> 1.5U/μg. Activity described as U=100 pmol/min at 25°C using a colorimetric assay with thiopeptide Ac-Pro-Leu-Gly-[2- mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol) as substrate.

Usage
Enzyme kinetic studies, cleavage of target substrates and screening of inhibitors.

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References
Velinov N., et al., Int J Biol Markers, 2007, 22(4):265-73.
Linksvan Horssen J., et al., Neuropathol Appl Neurobiol, 2006, 32(6):585-93.

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