MMP1cat

MMP1 catalytic domain

Human, recombinant.
residues 106-261, swissprot accession P03956
MW = 17600 Da.
EC # 3.4.24.7
CAT # G04MP01C

Packaging and prices - Technical Details - References

Packaging and Prices

Unlabeled Protein
Catalog n° Labeling Q.ty Price (€)
G04MP01C none 10 µg 200.00
G04MP01C none 5 x 10µg 400.00

Special prices for bulk quantities, or labelled samples, available upon request.

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Description
MW = 17.6k Da. Recombinant matrix metalloproteinase-1 (MMP-1, fibroblast collagenase, collagenase-1, interstitial collagenase) cloned from human cDNA, expressed in E. coli. The enzyme consists of the catalytic domain of human MMP-1 (residues 106-261, swissprot accession P03956).

Sequence
       110        120        130        140        150
   M-NPRWE QTHLTYRIEN YTPDLPRADV DHAIEKAFQL WSNVTPLTFT 
       160        170        180        190        200
KVSEGQADIM ISFVRGDHRD NSPFDGPGGN LAHAFQPGPG IGGDAHFDED 
       210        220        230        240        250
ERWTNNFREY NLHRVAAHEL GHSLGLSHST DIGALMYPSY TFSGDVQLAQ 
       260       
DDIDGIQAIY G
Purity
> 95% by SDS-PAGE. The protein was observed as 1 band migrating at a molecular weight of between 8.6 and 14 kDa.

Specific activity
> 100U/μg. Activity described as U=100 pmol/min at 25°C using a colorimetric assay with thiopeptide Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol) as substrate.

Usage
Enzyme kinetic studies, cleavage of target substrates and screening of inhibitors.

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