MMP9cat

MMP9 catalytic domain

Human, recombinant.
residues 112-445, swissprot accession P14780
MW = 37700 Da.
EC # 3.4.24.35
CAT # G04MP09C

Packaging and prices - Technical Details - References

Packaging and Prices

Unlabeled Protein
Catalog n° Labeling Q.ty Price (€)
G04MP09C none 10 µg 200.00
G04MP09C none 5 x 10 µg 400.00

Special prices for bulk quantities, or labelled samples, available upon request.

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Description
MW = 37.7 kDa. Recombinant Matrix Metalloproteinase-9 (MMP-9, 92 kDa type IV collagenase, 92 kDa gelatinase, Gelatinase B, GELB) catalytic domain with fibronectins domains cloned from human cDNA, expressed in E. coli. The recombinant enzyme consists of the catalytic domain of human MMP-9 (residues 112-445, Swiss prot accession P14780) with a N-term T7 tag.

Purity
> 90% by SDS-PAGE. In an SDS-PAGE gel, the enzyme runs as a single band migrating at a molecular weight of between 40.3 and 29.2 kDa.

Specific activity
> 10U/μg. Activity described as U=100 pmol/min at 25°C using a colorimetric assay with thiopeptide Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol) as substrate.

Usage
Enzyme kinetic studies, cleavage of target substrates and screening of inhibitors.

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References
G. Murphy and V. Knäuper Matrix Biol. 1997, 15, 511.
W. Bode et al. Cell. Mol. Life Sci. 1999, 55, 639.
M. Sawa et al. J. Med. Chem. 2002, 45, 930.
Ram M et al. J. Clin. Immunol 2006, 26, 299.

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