SH3

ALPHA-SPECTRIN - SH3

Fom chicken, recombinant, folding state checked by NMR.
residues 965-1025
MW = 7.2 kDa
CAT # G99SH301

Packaging and prices - Technical Details - References

Packaging and Prices

Unlabeled Protein
Catalog n° Labeling Q.ty Price (€)
G99SH301 none 1 mg 450.00
G99SH301 none 5 mg 1,000.00
G99SH301 none 10 mg 1,500.00

Labeled Protein
Catalog n° Labeling Q.ty Price (€)
G99SH301n 15N 5 mg 1,385.00
G99SH301n 15N 10 mg 2,460.00
G99SH301cn 13C15N 5 mg 2,770.00
G99SH301cn 13C15N 10 mg 4,615.00

Ready to use NMR standard
Catalog n° Labeling Tube Price (€)
G99SH301n_t3 15N 3 mm tube 925.00
G99SH301n_t5 15N 5 mm tube 1,845.00
G99SH301cn_t3 13C15N 3 mm tube 1,540.00
G99SH301cn_t5 13C15N 5 mm tube 3,385.00

Special prices for bulk quantities available upon request.

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Description
MW = 7.2kDa. Recombinant α-Spectrin domain SH3 (Spectrin, non-erythroid alpha chain, Fodrin alpha chain), cloned from cDNA from chicken and expressed in E.coli. The protein consists of the soluble SH3 domain of α-Spectrin from Gallus gallus (residues 965-1025). Swissprot accession P07751.

Sequence
                  970        980        990       1000
             M-DETGKE LVLALYDYQE KSPREVTMKK GDILTLLNST 
      1010       1020       
NKDWWKVEVN DRQGFVPAAY VKKLD
Purity
> 95% by SDS-PAGE. The protein was observed as a single band migrating at molecular weight <10 kDa.

Supplied as
Solution at concentration 1mM of protein in 10mM Sodium Citrate buffer pH 3.5, 0.02% NaN3, 10% D2O, sealed in NMR tube under argon conditions. The concentration is calculated from the absorbance at 280nm (ε280=15470 M-1cm-1).

Storage
NMR USE: after the experiments return the sample at 4°C.

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References
F.J. Blanco et al.J Biomol NMR. 1997 Jun;9(4):347-57

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