Ubiquitin

Ubiquitin

Human, recombinant, folding state checked by NMR.
residues 1-76
MW = 8.5 kDa
CAT # G03UBQ01

Packaging and prices - Technical Details - References

Packaging and Prices

Unlabeled Protein
Catalog n° Labeling Q.ty Price (€)
G03UBQ01 none 1 mg 200.00
G03UBQ01 none 5 mg 480.00
G03UBQ01 none 10 mg 700.00
G03UBQ01 none 20 mg 1,050.00

Labeled Protein
Catalog n° Labeling Q.ty Price (€)
G03UBQ01n 15N 5 mg 615.00
G03UBQ01n 15N 10 mg 1,230.00
G03UBQ01cn 13C15N 5 mg 925.00
G03UBQ01cn 13C15N 10 mg 1,695.00
G03UBQ01dcn 2H13C15N 5 mg 3,080.00
G03UBQ01dcn 2H13C15N 10 mg 4,450.00

Ready to use NMR standard
Catalog n° Labeling Tube Price (€)
G03UBQ01n_t3 15N 3 mm tube 385.00
G03UBQ01n_t5 15N 5 mm tube 845.00
G03UBQ01cn_t3 13C15N 3 mm tube 540.00
G03UBQ01cn_t5 13C15N 5 mm tube 1,230.00
G03UBQ01dcn_t3 2H13C15N 3 mm tube 1,540.00
G03UBQ01dcn_t5 2H13C15N 5 mm tube 3,390.00

Labeled microcrystals
Catalog n° Labeling Q.ty Price (€)
G03UBQ02cn 13C15N 5 mg 1,080.00
G03UBQ02cn 13C15N 10 mg 1,950.00
G03UBQ02dcn 2H13C15N 5 mg 3,380.00
G03UBQ02dcn 2H13C15N 10 mg 4,770.00

Special prices for bulk quantities available upon request.

(▲ Top)

Description
MW= 8.6 kDa. Full length Ubiquitin cloned from human cDNA, expressed in E. coli. The protein consists of the human ubiquitin (residues 1-76, swissprot accession P0CG47).

Sequence
        10         20         30         40         50
MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL 
        60         70        
EDGRTLSDYN IQKESTLHLV LRLRGG
Purity
> 95% by SDS-PAGE. The protein was observed as a single band migrating at molecular weight <10 kDa.

Supplied as
1mg/mL in Phosphate buffer 50mM, pH 7, NaN3 0.02%. The concentration is calculated from the absorbance at 280nm (ε280=1490 M-1cm-1).

Characteristics
Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated up to 2mM.

Storage
-20°C. The protein is stable at 4°C for months.
Avoid repeated freeze/defrost cycles.
NMR USE: after the experiments return the sample at 4°C.

(▲ Top)

References
A. Hershko, and A. Ciechnover Annu Rev Biochem (1998); 67: 425-79
R.Z. Orlowski Cell Death Differ. (1999) 6 (4): 303-13
J.H. Hurley, Biochem J. (2006) 1; 399(3): 361-72

(▲ Top)