Description
Description
MW= 140 kDa calculated (tetramer). L-Asparaginase II from E. coli (ANSII) consists of a homotetrameric assembly of 140kDa with D2 symmetry. (residues 7-348, UniProtKB accession P00805).
Sequence
10 20 30 40 50
TALA ALVMGFSGAA LALPNITILA TGGTIAGGGD SATKSNYTVG
60 70 80 90 100
KVGVENLVNA VPQLKDIANV KGEQVVNIGS QDMNDNVWLT LAKKINTDCD
110 120 130 140 150
KTDGFVITHG TDTMEETAYF LDLTVKCDKP VVMVGAMRPS TSMSADGPFN
160 170 180 190 200
LYNAVVTAAD KASANRGVLV VMNDTVLDGR DVTKTNTTDV ATFKSVNYGP
210 220 230 240 250
LGYIHNGKID YQRTPARKHT SDTPFDVSKL NELPKVGIVY NYANASDLPA
260 270 280 290 300
KALVDAGYDG IVSAGVGNGN LYKSVFDTLA TAAKTGTAVV RSSRVPTGAT
310 320 330 340
TQDAEVDDAK YGFVASGTLN PQKARVLLQL ALTQTKDPQQ IQQIFNQY
Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at molecular weight at about 35.0 kDa.
Supplied as
1 mg/mL solution in phosphate buffer 150 mM pH 7.5. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 23505 M-1cm-1 calculated for the monomer).
(unless otherwise specified, NMR tubes will be sold at a concentration of 1mM)
Characteristics
Under the above described conditions, the product can be concentrated up to 3 mM.
Storage
-20°C. The protein is stable at 4°C for months. Avoid repeated freeze/thaw cycles.
NMR USE: after the experiments return the sample at 4°C.
References
E. Ravera, S. Ciambellotti, L. Cerofolini, T. Martelli, T. Kozyreva, C. Bernacchioni, S. Giuntini, M. Fragai, P. Turano, C. Luchinat, Angew. Chem. Int. Ed. Engl. 2016,
55, 2446–2449.
L. Cerofolini, S. Giuntini, A. Carlon, E. Ravera, V. Calderone, M. Fragai, G. Parigi, C. Luchinat, Chemistry 2019, 25, 1984–1991.
S. Giuntini, L. Cerofolini, E. Ravera, M. Fragai, C. Luchinat, Sci Rep 2017, 7, 17934
S. Giuntini, E. Balducci, L. Cerofolini, E. Ravera, M. Fragai, F. Berti, C. Luchinat, Angew. Chem. Int. Ed. 2017, 56, 14997–15001.
L. Cerofolini, S. Giuntini, E. Ravera, C. Luchinat, F. Berti, M. Fragai, npj Vaccines 2019, 4, 20
