NSP9

Description

DESCRIPTION
MW= 13.6 kDa. Full length nsp9 from SARS-CoV-2, expressed in E. coli. The protein consists in residues 1-198 (NCBI reference sequence YP_009725305.1). An additional his-tag is present at the C-term of the construct (highlighted in italic in the scheme below).

SEQUENCE
Numeration of the nsp9 from NCBI YP_009725305.1

                   10         20         30         40         50 
        M- NNELSPVALR QMSCAAGTTQ TACTDDNALA YYNTTKGGRF VLALLSDLQD 
        60         70         80         90        100        110
DLKWARFPKS GTGTIYTELE PPCRFVTDTP KGPKVKYLYF IKGLNNLNRG MVLGSLAATV

RLQ LEHHHHHH 

ISOTOPIC ENRICHMENT
none

SUPPLIED AS
Protein solution. The concentration is calculated from the absorbance at 280nm (ε₂₈₀ = 13075 M^-1cm^-1).

STORAGE
-20°C. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.

REFERENCES
Dong, S. et al. J. Med. Virol. (March), 1–7 (2020). doi:10.1002/jmv.25768
Miknis, Z. J. et al. J. Virol. 83 (7), 3007–3018 (2009).