GB1

5.060,00

Recombinant, from Streptococcus sp
Residues 303-357, T303Q mutant, UniProtKB accession P19909
MW = 6.2 kDa
CAT # G03GB101

Add120,00840,00
Add250,001.755,00
Add490,001.220,00
Add350,002.455,00
Add620,001.635,00
Add2.200,004.400,00
Add1.370,002.265,00
Add820,002.015,00
Add1.145,002.820,00
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Add2.530,005.060,00
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Catalog n.Physical stateLabelingNMR StandardQtyPrice
120,00
340,00
535,00
840,00
250,00
715,00
1.120,00
1.755,00
490,00
840,00
1.220,00
350,00
1.000,00
1.565,00
2.455,00
620,00
1.105,00
1.635,00
2.200,00
3.115,00
4.400,00
1.370,00
2.265,00
820,00
1.285,00
2.015,00
1.145,00
1.800,00
2.820,00
Request a quote
Request a quote
2.530,00
3.580,00
5.060,00
Request a quote
Request a quote
VAT not included

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Description

Description
MW = 6.2 kDa calculated. GB1 (Immunoglobulin G-binding protein G, IgG-binding protein G) expressed in E. coli. The protein consists β-1 Immunoglobulin Binding Domain of Protein G, residues 303-357 (UniProtKB accession P19909), with the mutation T303Q (T2Q). This mutation reduces the cleavage of the N-terminal Met residue.

Sequence

       310        320        330        340        350
M-QYKLILNG KTLKGETTTE AVDAATAEKV FKQYANDNGV DGEWTYDDAT 
       
KTFTVTE

Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at molecular weight below 14.4 kDa.

Supplied as
1 mg/mL solution in sodium phosphate 20 mM, pH 7. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280= 9970 M-1cm-1 calculated).

Characteristics
Under the above described conditions, to avoid precipitation or protein aggregation, the product can be concentrated up to 3 mM.

Storage
-20°C. The protein is stable at 4°C for months at 25°C for at least two weeks. Avoid repeated freeze/thaw cycles.
NMR USE: after the experiments return the sample at 4°C.

 
References
Frericks Schmidt, H.L. et al. J. Phys. Chem. B 111 (51), 14362-14369 (2007).
Franks, W.T. et al. J. Am. Chem. Soc. 127 (35), 12291-12305 (2005).

Additional information

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