MMP1 - catalytic domain

200,00400,00

Human, recombinant
Residues 106-261, UniProtKB accession P03956
MW = 17.6 kDa
EC # 3.4.24.7
CAT # G04MP01C

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SKU: G04MP01C Categories: , Tags: , , , ,
Catalog n.QtyPrice
200,00
400,00
VAT not included

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Description

Description
MW = 17.6 kDa calculated. Recombinant matrix metalloproteinase-1 (MMP-1, fibroblast collagenase, collagenase-1, interstitial collagenase) cloned from human cDNA, expressed in E. coli. The enzyme consists of the catalytic domain of human MMP-1 (residues 106-261, UniProtKB accession P03956).
 
Sequence

       110        120        130        140        150
   M-NPRWE QTHLTYRIEN YTPDLPRADV DHAIEKAFQL WSNVTPLTFT 
       160        170        180        190        200
KVSEGQADIM ISFVRGDHRD NSPFDGPGGN LAHAFQPGPG IGGDAHFDED 
       210        220        230        240        250
ERWTNNFREY NLHRVAAHEL GHSLGLSHST DIGALMYPSY TFSGDVQLAQ 
       260       
DDIDGIQAIY G

Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight between 14.4 and 18.4 kDa.

Supplied as
0.2 mg/mL solution in Tris 20 mM, pH 7.2, CaCl2 10 mM, ZnCl2 0.1 mM, NaCl 0.3 M, acetohydroxamic acid (AHA) 0.2 M. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280= 28420 M-1cm-1 calculated).

Storage
-80°C. After initial defrost, aliquot the product into individual tubes and refreeze at -80°C.
Avoid repeated freeze/thaw cycles.

Specific activity
> 100U/μg. Activity described as U = 100 pmol/min at 25°C using a colorimetric assay with thiopeptide Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol) as substrate.

Usage
Enzyme kinetic studies, cleavage of target substrates and screening of inhibitors.

References

Additional information

Qty

10 μg, 5 x 10 μg

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