MMP12 - hemopexin like domain

200,00400,00

Human, recombinant
Residues 278-470, UniProtKB accession P39900
MW = 23.2 kDa
EC # 3.4.24.7
CAT # G04MP12H

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SKU: G04MP12H Categories: , Tags: , , , , ,
Catalog n.QtyPrice
200,00
400,00
VAT not included

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Description

Description
MW = 23.2 kDa calculated. Hemopexin-Like domain of MMP-12 (Macrophage metalloelastase, Matrix metalloproteinase 12), cloned from human cDNA, expressed in E. coli. The protein consists of the hemopexin-like domain of human MMP-12 (residues 278-470, UniProtKB accession P39900 ).
 
Sequence
       280        290        300        310        320
     M-EPA LCDPNLSFDA VTTVGNKIFF FKDRFFWLKV SERPKTSVNL
       330        340        350        360        370 
ISSLWPTLPS GIEAAYEIEA RNQVFLFKDD KYWLISNLRP EPNYPKSIHS
       380        390        400        410        420 
FGFPNFVKKI DAAVFNPRFY RTYFFVDNQY WRYDERRQMM DPGYPKLITK
       430        440        450        460        470
NFQGIGPKID AVFYSKNKYY YFFQGSNQFE YDFLLQRITK TLKSNSWFGC
 
Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight between 18.4 and 25.0 kDa.
 
Supplied as
0.1 mg/mL solution in Tris 20 mM pH 7.2, CaCl2 10 mM, ZnCl2 0.1 mM, NaCl 150 mM. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 46995 M-1cm-1 calculated).
 
Storage
-80°C. After initial defrost, aliquot the product into individual tubes and refreeze at -80°C.
Avoid repeated freeze/thaw cycles.
 
Usage
Enzyme kinetic studies, cleavage of target substrates and screening of inhibitors.
 
References
Bertini I., J Am Chem Soc. 2008;130(22):7011-21.

Additional information

Qty

10 μg, 5 x 10 μg

Shipping in Dry Ice

yes

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