MMP14 - catalytic domain, mutant with improved stability

200,00400,00

Human, recombinant
Residues 114-290, C127S mutant, UniProtKB accession P50281
MW = 20.1 kDa.
EC # 3.4.24.80
CAT # G04MP14Cm

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SKU: G04MP14Cm Categories: , Tags: , , , , , ,
Catalog n.QtyPrice
200,00
400,00
VAT not included

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Description

Description
MW = 20.1 kDa calculated. Recombinant Matrix Metalloproteinase-14 (MMP-14, Membrane-Type Matrix Metalloproteinase1, MT1- MMP) cloned from human cDNA, expressed in E. coli. The enzyme consists of the catalytic domain of human MMP-14 (residues 114-290, UniProtKB accession P50281). The protein has the mutation C127S to increase its stability. The catalytic activity rates are not affected by the mutation.
 
Sequence
       120        130        140        150        160
   IQGLKWQ HNEITFSIQN YTPKVGEYAT YEAIRKAFRV WESATPLRFR 
       170        180        190        200        210
EVPYAYIREG HEKQADIMIF FAEGFHGDST PFDGEGGFLA HAYFPGPNIG
       220        230        240        250        260
GDTHFDSAEP WTVRNEDLNG NDIFLVAVHE LGHALGLEHS SDPSAIMAPF
       270        280        290 
YQWMDTENFV LPDDDRRGIQ QLYGGESGFP
 
Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight between 18.8 and 25.0 kDa.
 
Supplied as
0.2 mg/mL solution in Tris 20 mM pH 7.2, CaCl2 10 mM, ZnCl2 0.1 mM, NaCl 0.3 M, acetohydroxamic acid (AHA) 0.5 M. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 33920 M-1cm-1 calculated).

Specific activity
> 150 U/μg. Activity described as U=100 pmol/min at 25°C using a colorimetric assay with thiopeptide Ac-Pro-Leu-Gly-[2- mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol) as substrate.

Storage
-80°C. After initial defrost, aliquot the product into individual tubes and refreeze at -80°C.
Avoid repeated freeze/thaw cycles.

Usage
Enzyme kinetic studies, cleavage of target substrates and screening of inhibitors.

References
M. Gioia et al. J Mol Biol. 2007 May 11;368(4):1101-13.
K. Lehti et al. J. Biol. Chem. 2000, 275:15006-13.
G. Murphy and V. Knäuper Matrix Biol. 1997, 15, 511.
W. Bode et al. Cell Mol Life Sci 1999, 55:639-52.

Additional information

Qty

10 μg, 5 x 10 μg

Shipping in Dry Ice

yes

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