MMP2 - catalytic domain fibronectin deficient

Description
MW = 17.5 kDa calculated. Matrix Metalloproteinase-2 (MMP-2, Gelatinase A, Type IV collagenase) catalytic domain without fibronectin domains cloned from human cDNA, expressed in E. coli. The enzyme consists of the catalytic domain of human MMP-2 fibronectin deficient (residues 115-214 and 393-447 where the residue numbers are based on the unprocessed precursor, UniProtKB accession P08253).

Sequence

                  120        130        140        150
             M-RKPKWD KNQITYRIIG YTPDLDPETV DDAFARAFQV 
       160        170        180        190        200
WSDVTPLRFS RIHDGEADIM INFGRWEHGD GYPFDGKDGL LAHAFAPGTG 
       210     
VGGDSHFDDD ELWT                                    400
                                              QGYSLFLV 
       410        420        430        440     447
AAHEFGHAMG LEHSQDPGAL MAPIYTYTKN FRLSQDDIKG IQELYGA

Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight between 14.4 and 18.4 kDa.

Supplied as
0.15 mg/mL solution in Tris 20 mM pH 7.2, CaCl₂ 10 mM, ZnCl₂ 0.1 mM, NaCl 0.3 M, acetohydroxamic acid (AHA) 0.5 M, glycerol 10%, Brij-35 0.05%. The concentration is calculated by the analysis of the absorbance at 280 nm (ε₂₈₀= 32430 M^-1cm^-1 calculated).

Specific activity
> 40U/μg. Activity described as U=100 pmol/min at 25°C using a colorimetric assay with thiopeptide Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OC₂H₅ (Biomol) as substrate.

Storage
-80°C. After initial defrost, aliquot the product into individual tubes and refreeze at -80°C.
Avoid repeated freeze/thaw cycles.

Usage
Enzyme kinetic studies, cleavage of target substrates and screening of inhibitors.

RELATED RESEARCH FIELDS

• Biomaterials
• Cancer
  • breast cancer
  • lung cancer
  • pancreatic cancer
  • prostate cancer
• Cardiovascular disorders
  • aneurysm formation
  • coronary artery disease
  • myocardial infarction
• Dental diseases
  • caries
  • periodontitis
• Diabetes Mellitus
• Neurodegenerative diseases
  • Alzheimer’s disease
  • Amyotrophic lateral sclerosis
  • Multiple sclerosis
  • Parkinson’s disease
• Renal pathologies
  • chronic allograft nephropathy
  • diabetic nephropathy
  • glomerulosclerosis/tubulointerstitial fibrosis
  • polycystic kidney disease
  • renal cell carcinoma
• Tissue regeneration
• Wound healing

Cheng D. et al. Protein Expr Purif. 2003 Jan; 27(1):63-74
Steffensen B. at al. J Biol Chem. 1995 May 12; 270(19):11555-66
Banyai L. et al. FEBS Lett. 1991 Apr 22; 282(1):23-5.