MMP2 - hemopexin like domain

Description
MW = 22.4 kDa calculated. Hemopexin-like domain (C domain) of MMP-2, cloned from human cDNA, expressed in E. coli. The enzyme consists of the hemopexin-like domain of human MMP-2 (residues 467-660, UniProtKB accession P08253).

Sequence

                  470        480        490        500
               M-EICK QDIVFDGIAQ IRGEIFFFKD RFIWRTVTPR 
       510        520        530        540        550
DKPMGPLLVA TFWPELPEKI DAVYEAPQEE KAVFFAGNEY WIYSASTLER 
       560        570        580        590        600
GYPKPLTSLG LPPDVQRVDA AFNWSKNKKT YIFAGDKFWR YNEVKKKMDP 
       610        620        630        640        650
GFPKLIADAW NAIPDNLDAV VDLQGGGHSY FFKGAYYLKL ENQSLKSVKF 
       660
GSIKSDWLGC

Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight between 18.4 and 25.0 kDa.

Supplied as
0.25 mg/mL in KPi 20mM pH 7.0, NaCl 250mM. The concentration is calculated from the absorbance at 280nm (ε₂₈₀ = 52035 M^-1cm^-1).

Storage
-80°C. After initial defrost, aliquot the product into individual tubes and refreeze at -80°C.
Avoid repeated freeze/thaw cycles.

Usage
Interaction studies.

RELATED RESEARCH FIELDS

U.M. Wallon and C.M.Overall. J Biol. Chem. 272(11), 7473-7481 (1997).