MW = 22.4 kDa calculated. Hemopexin-like domain (C domain) of MMP-2, cloned from human cDNA, expressed in E. coli. The enzyme consists of the hemopexin-like domain of human MMP-2 (residues 467-660, UniProtKB accession P08253).
470 480 490 500 M-EICK QDIVFDGIAQ IRGEIFFFKD RFIWRTVTPR 510 520 530 540 550 DKPMGPLLVA TFWPELPEKI DAVYEAPQEE KAVFFAGNEY WIYSASTLER 560 570 580 590 600 GYPKPLTSLG LPPDVQRVDA AFNWSKNKKT YIFAGDKFWR YNEVKKKMDP 610 620 630 640 650 GFPKLIADAW NAIPDNLDAV VDLQGGGHSY FFKGAYYLKL ENQSLKSVKF 660 GSIKSDWLGC
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight between 18.4 and 25.0 kDa.
0.25 mg/mL in KPi 20mM pH 7.0, NaCl 250mM. The concentration is calculated from the absorbance at 280nm (ε280 = 51350 M-1cm-1).
-80°C. After initial defrost, aliquot the product into individual tubes and refreeze at -80°C.
Avoid repeated freeze/thaw cycles.
Enzyme kinetic studies, cleavage of target sbstrates and screening of inhibitors.