MMP7 - catalytic domain

200,00400,00

Human, recombinant
Residues 95-267, UniProtKB accession P09237
MW = 19.3 kDa
EC # 3.4.24.23
CAT # G04MP07C

Clear
SKU: G04MP07C Categories: , Tags: , , , , ,
Catalog n.QtyPrice
200,00
400,00
VAT not included

For any special request or bulk quantities Click Here

Description

Description
MW = 19.3 kDa calculated. Recombinant Matrix Metalloproteinase-7 (MMP-7, Matrilysin, Uterine metalloproteinase) cloned from human cDNA, expressed in E. coli. The enzyme consists of the catalytic domain of human MMP-7 (residues 95-267, UniProtKB accession P09237).
 
Sequence
       100        110        120        130        140
  M-YSLFPN SPKWTSKVVT YRIVSYTRDL PHITVDRLVS KALNMWGKEI
       150        160        170        180        190 
PLHFRKVVWG TADIMIGFAR GAHGDSYPFD GPGNTLAHAF APGTGLGGDA 
       200        210        220        230        240
HFDEDERWTD GSSLGINFLY AATHELGHSL GMGHSSDPNA VMYPTYGNGD 
       250        260
PQNFKLSQDD IKGIQKLYGK RSNSRKK
 
Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight between 18.8 kDa and 25.0 kDa.

Supplied As
0.2 mg/mL solution in Tris 20 mM pH 7.2, CaCl2 10 mM, ZnCl2 0.1 mM, NaCl 0.3 M, acetohydroxamic acid (AHA) 0.5 M. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 33920 M-1cm-1 calculated).

Specific activity
> 8U/μg. Activity described as U=100 pmol/min at 25°C using a colorimetric assay with thiopeptide Ac-Pro-Leu-Gly-[2- mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol) as substrate.

Storage
-80°C. After initial defrost, aliquot the product into individual tubes and refreeze at -80°C.
Avoid repeated freeze/thaw cycles.

Usage
Enzyme kinetic studies, cleavage of target substrates and screening of inhibitors.

References
Ou, L. et al. J. Biomol. NMR 29 (4), 537-538 (2004).
McCawley, L.J. & Matrisian, L.M. Curr. Opin. Cell Biol. 13 (5), 534-540 (2001).
Stetler-Stevenson, W.G. & Yu, A.E. Semin. Cancer Biol. 11 (2), 143-152 (2001).
Browner, M.F., Smith, W.W. & Castelhano, A.L. Biochemistry 34 (20), 6602-6610 (1995).

Additional information

Qty

10 μg, 5 x 10 μg

Shipping in Dry Ice

yes

Social media & sharing icons powered by UltimatelySocial

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this.

Close