MMP9 - catalytic domain

200,00400,00

Human, recombinant
Residues 112-445, UniProtKB accession P14780
MW = 37.7 kDa
EC # 3.4.24.35
CAT # G04MP09C

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SKU: G04MP09C Categories: , Tags: , , , , ,
Catalog n.QtyPrice
200,00
400,00
VAT not included

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Description

Description
MW = 37.7 kDa calculated. Recombinant Matrix Metalloproteinase-9 (MMP-9, 92 kDa type IV collagenase, 92 kDa gelatinase, Gelatinase B, GELB) catalytic domain with fibronectin domains cloned from human cDNA, expressed in E. coli. The recombinant enzyme consists of the catalytic domain of human MMP-9 (residues 112-445, UniProtKB accession P14780) with a N-term T7 tag.
 
Sequence
       120        130        140        150        160
   MGRGSEF -GDLKWHHHN ITYWIQNYSE DLPRAVIDDA FARAFALWSA
       170        180        190        200        210 
VTPLTFTRVY SRDADIVIQF GVAEHGDGYP FDGKDGLLAH AFPPGPGIQG
       220        230        240        250        260 
DAHFDDDELW SLGKGVVVPT RFGNADGAAC HFPFIFEGRS YSACTTDGRS 
       270        280        290        300        310
DGLPWCSTTA NYDTDDRFGF CPSERLYTQD GNADGKPCQF PFIFQGQSYS
       320        330        340        350        360  
ACTTDGRSDG YRWCATTANY DRDKLFGFCP TRADSTVMGG NSAGELCVFP
       370        380        390        400        410
FTFLGKEYST CTSEGRGDGR LWCATTSNFD SDKKWGFCPD QGYSLFLVAA
       420        430        440        450 
HEFGHALGLD HSSVPEALMY PMYRFTEGPP LHKDDVNGIR HLYGP
 
Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight between 35.0 and 45.0 kDa.
 
Supplied as
0.15 mg/mL solution in Tris 20 mM pH 7.2, CaCl2 5 mM, ZnCl2 0.1 mM, NaCl 0.3 M, acetohydroxamic acid (AHA) 0.5 M, glycerol 10%, Brij35 0.05%. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 67100 M-1cm-1 calculated).

Specific activity
> 10 U/μg. Activity described as U=100 pmol/min at 25°C using a colorimetric assay with thiopeptide Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol) as substrate.

Storage
-80°C. After initial defrost, aliquot the product into individual tubes and refreeze at -80°C.
Avoid repeated freeze/thaw cycles.

Usage
Enzyme kinetic studies, cleavage of target substrates and screening of inhibitors.

References
Ram, M., Sherer, Y. & Shoenfeld, Y. J. Clin. Immunol. 26 (4), 299-307 (2006).
Sawa, M. et al. J. Med. Chem. 45 (4), 930-936 (2002).
Bode, W. et al. Cell. Mol. Life Sci. 55 (4), 639-652 (1999).
Murphy, G. & Knäuper, V. Matrix Biol. 15 (8-9), 511-518 (1997).

Additional information

Qty

10 μg, 5 x 10 μg

Shipping in Dry Ice

yes

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