NSP14

Description

DESCRIPTION
MW= 59.8 kDa. Full length nsp14 (3′-to-5′ exonuclease) from SARS-CoV-2, expressed in E. coli. The protein consists in residues 1-527 (NCBI reference sequence YP_009725309.1).

SEQUENCE
Numeration of the nsp14 from NCBI YP_009725309.1

        10         20         30         40         50         60 
AENVTGLFKD CSKVITGLHP TQAPTHLSVD TKFKTEGLCV DIPGIPKDMT YRRLISMMGF 
        70         80         90        100        110        120 
KMNYQVNGYP NMFITREEAI RHVRAWIGFD VEGCHATREA VGTNLPLQLG FSTGVNLVAV 
       130        140        150        160        170        180 
PTGYVDTPNN TDFSRVSAKP PPGDQFKHLI PLMYKGLPWN VVRIKIVQML SDTLKNLSDR 
       190        200        210        220        230        240 
VVFVLWAHGF ELTSMKYFVK IGPERTCCLC DRRATCFSTA SDTYACWHHS IGFDYVYNPF 
       250        260        270        280        290        300 
MIDVQQWGFT GNLQSNHDLY CQVHGNAHVA SCDAIMTRCL AVHECFVKRV DWTIEYPIIG 
       310        320        330        340        350        360 
DELKINAACR KVQHMVVKAA LLADKFPVLH DIGNPKAIKC VPQADVEWKF YDAQPCSDKA 
       370        380        390        400        410        420 
YKIEELFYSY ATHSDKFTDG VCLFWNCNVD RYPANSIVCR FDTRVLSNLN LPGCDGGSLY 
       430        440        450        460        470        480 
VNKHAFHTPA FDKSAFVNLK QLPFFYYSDS PCESHGKQVV SDIDYVPLKS ATCITRCNLG 
       490        500        510        520 
GAVCRHHANE YRLYLDAYNM MISAGFSLWV YKQFDTYNLW NTFTRLQ 

ISOTOPIC ENRICHMENT
none

SUPPLIED AS
Protein solution. The concentration is calculated from the absorbance at 280nm (ε₂₈₀ = 93625 M^-1cm^-1).

STORAGE
-20°C. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.

REFERENCES
Dong, S. et al.    J. Med. Virol. (March), 1–7 (2020).
Ma, Y. et al. Proc. Natl. Acad. Sci. U. S. A. 112 (30), 9436–9441 (2015).