NSP3, papain-like proteinase domain

Description

DESCRIPTION
MW= 36.8 kDa. 746-1060 domain of nsp3 (papain-like proteinase, nsp3d, Plpro) from SARS-CoV-2, expressed in E. coli. The protein consists in residues 746-1060 (NCBI reference sequence YP_009725299.1) with an additional C-term his-tag (highlighted in italic in the scheme below).

SEQUENCE
Numeration of the nsp3 from NCBI YP_009725299.1

          750        760        770        780        790        800 
   M-EVRTI KVFTTVDNIN LHTQVVDMSM TYGQQFGPTY LDGADVTKIK PHNSHEGKTF
       810        820        830        840        850        860
YVLPNDDTLR VEAFEYYHTT DPSFLGRYMS ALNHTKKWKY PQVNGLTSIK WADNNCYLAT
       870        880        890        900        910        920
ALLTLQQIEL KFNPPALQDA YYRARAGEAA NFCALILAYC NKTVGELGDV RETMSYLFQH         
       930        940        950        960        970        980
ANLDSCKRVL NVVCKTCGQQ QTTLKGVEAV MYMGTLSYEQ FKKGVQIPCT CGKQATKYLV
       990       1000       1010       1020       1030       1040
QQESPFVMMS APPAQYELKH GTFTCASEYT GNYQCGHYKH ITSKETLYCI DGALLTKSSE
      1050       1060
YKGPITDVFY KENSYTTTIK LEHHHHHH 

ISOTOPIC ENRICHMENT
none

SUPPLIED AS
Protein solution. The concentration is calculated from the absorbance at 280nm (ε₂₈₀ = 45895 M^-1cm^-1).

STORAGE
-20°C. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.

REFERENCES
Dong, S. et al.    J. Med. Virol. (March), 1–7 (2020). doi:10.1002/jmv.25768
Frieman, M. et al.           J. Virol. 83 (13), 6689–6705 (2009).
Wang, L., Hu, W. & Fan, C.          Protein Sci. (March), 1–14 (2020). doi:10.1002/pro.3857