NSP5

Description

DESCRIPTION
MW= 34.2 kDa. Full length nsp5 (main protease, 3CLp, 3CL-PRO, 3C-like proteinase) from SARS-CoV-2, expressed in E. coli. The protein consists in residues 1-306 (NCBI reference sequence YP_009725301.1). Four additional residues, GSHM, are present at the N-term of the protein.

SEQUENCE
Numeration of the nsp5 from NCBI YP_009725301.1

                   10         20         30         40         50 
      GSHM SGFRKMAFPS GKVEGCMVQV TCGTTTLNGL WLDDVVYCPR HVICTSEDML 
        60         70         80         90        100        110 
NPNYEDLLIR KSNHNFLVQA GNVQLRVIGH SMQNCVLKLK VDTANPKTPK YKFVRIQPGQ 
       120        130        140        150        160        170 
TFSVLACYNG SPSGVYQCAM RPNFTIKGSF LNGSCGSVGF NIDYDCVSFC YMHHMELPTG 
       180        190        200        210        220        230 
VHAGTDLEGN FYGPFVDRQT AQAAGTDTTI TVNVLAWLYA AVINGDRWFL NRFTTTLNDF 
       240        250        260        270        280        290 
NLVAMKYNYE PLTQDHVDIL GPLSAQTGIA VLDMCASLKE LLQNGMNGRT ILGSALLEDE 
       300 
FTPFDVVRQC SGVTFQ 

ISOTOPIC ENRICHMENT
none

SUPPLIED AS
Protein solution. The concentration is calculated from the absorbance at 280nm (ε₂₈₀ = 33640 M^-1cm^-1).

STORAGE
-20°C. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.

REFERENCES
Dong, S. et al.    J. Med. Virol. (March), 1–7 (2020).
Jin, Z. et al.         Nature 1–9 (2020). doi:10.1038/s41586-020-2223-y
Xue, X. et al.      J. Mol. Biol. 366 (3), 965–975 (2007).