MW = 22.9 kDa (dimer) calculated. Full length S100A13 (S100 Calcium Binding Protein A13) cloned from human cDNA, expressed in E. coli. The protein consists of the human S100A13 (residues 1-98, UniProtKB accession Q99584).
10 20 30 40 50 MAAEPLTELE ESIETVVTTF FTFARQEGRK DSLSVNEFKE LVTQQLPHLL 60 70 80 90 KDVGSLDEKM KSLDVNQDSE LKFNEYWRLI GELAKEIRKK KDLKIRKK
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as single band (monomer) migrating at a molecular weight below 14.4 kDa.
0.5 mg/mL solution in sodium acetate 50 mM pH 5.6. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280= 6970 M-1cm-1 calculated).
-20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.
Arnesano, F. et al. Angew. Chemie Int. Ed. 44 (39), 6341-6344 (2005).
Wicki, R. et al. Biochem. Biophys. Res. Commun. 227, 594-599 (1996).