MW = 22.0 kDa (dimer) calculated. Full length S100A2 (S100L, S100 calcium binding protein A2) cloned from human cDNA, expressed in E. coli. The protein consists of the human S100A2 (residues 1-98, UniProtKB accession P29034). The starting methionine is processed during the expression.
10 20 30 40 50 MMCSSLEQAL AVLVTTFHKY SCQEGDKFKL SKGEMKELLH KELPSFVGEK 60 70 80 90 VDEEGLKKLM GSLDENSDQQ VDFQEYAVFL ALITVMCNDF FQGCPDRP
> 95% by SDS-PAGE. The protein is observed, in denaturating conditions, as two bands representing the monomer, migrating at a molecular weight below 14.4 kDa, and the dimer, migrating at a molecular weight between 18.4 and 25.0 kDa.
1 mg/mL solution in Tris 50 mM, pH 7.5, CaCl2 5 mM, DTT 2 mM. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 2980 M-1cm-1 calculated).
-20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.
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