MW = 23.4 kDa (dimer) calculated. Full length S100A4 (S100 calcium-binding protein A4, Calvasculin, Metastasin, Placental calcium-binding protein, Protein Mts1) cloned from human cDNA, expressed in E. coli. The protein consists of the human S100A4 (residues 1-101, UniProtKB accession P26447).
10 20 30 40 50 MACPLEKALD VMVSTFHKYS GKEGDKFKLN KSELKELLTR ELPSFLGKRT 60 70 80 90 100 DEAAFQKLMS NLDSNRDNEV DFQEYCVFLS CIAMMCNEFF EGFPDKQPRK K
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as single band (monomer) migrating at a molecular weight below 14.4 kDa.
1 mg/mL solution in Tris 20 mM, pH 7.5, NaCl 150 mM, CaCl2 5 mM, DTT 2 mM. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 2980 M-1cm-1 calculated).
-20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.
Engelkamp D., et al. Biochemistry 31:10258-10264(1992)
Vallely K.M., et al. Biochemistry 41:12670-12680 (2002)
Boye K, et al. Am J Pathol. Feb;176(2):528-35(2010)