MW = 21.5 kDa (dimer) calculated. Full length S100A5 (S100 calcium binding protein A5, S100D) cloned from human cDNA, expressed in E. coli. The protein consists of the human S100A5 (residues 1-92, UniProtKB accession P33763).
10 20 30 40 50 METPLEKALT TMVTTFHKYS GREGSKLTLS RKELKELIKK ELCLGEMKES 60 70 80 90 SIDDLMKSLD KNSDQEIDFK EYSVFLTMLC MAYNDFFLED NK
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as single band (monomer) migrating at a molecular weight below 14.4 kDa.
1 mg/mL solution in Tris 20 mM, pH 7.5, NaCl 150 mM. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 4595 M-1cm-1 calculated).
-20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.
Bertini, I. et al. JBIC J. Biol. Inorg. Chem. 14 (7), 1097-1107 (2009).
Carafoli, E. & Brini, M. Subcell. Biochem., Calcium Signalling and Disease,45 (Springer Netherlands: Dordrecht, 2007).
Schafer, B.W. et al. J. Biol. Chem. 275 (39), 30623-30630 (2000).