S100A7

Description
MW = 23.7 kDa (dimer) calculated. Full length S100A7 (S100 calcium-binding protein A7, psoriasin) cloned from human DNA expressed in E. coli. The protein consists of the human S100A7 natural variant E28D (UniProtKB accession Q5SY67). Four additional residues, GSFT, are present at N-Term of the protein.

 

Sequence

             10         20         30         40         50
GSFT-MSNTQAERSI IGMIDMFHKY TRRDDKIEKP SLLTMMKENF PNFLSACDKK 
             60         70         80         90        100
     GTNYLADVFE KKDKNEDKKI DFSEFLSLLG DIATDYHKQS HGAAPCSGGS 

     Q

Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as single band (monomer) migrating at a molecular weight below 14.4 kDa.

Supplied as
2 mg/mL solution in MES 30 mM, pH 6.5, KCl 150 mM, CaCl₂ 1 mM. The concentration is calculated by the analysis of the absorbance at 280 nm (ε₂₈₀ = 4400 M^-1cm^-1 calculated).

Storage
-20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.

References
Brodersen, D.E., Nyborg, J. & Kjeldgaard, M. Biochemistry 38 (6), 1695-1704 (1999).
Burgisser, D.M. et al. Biochem. Biophys. Res. Commun. 217 (1), 257-263 (1995).