MW = 21,6 kDa (dimer). Full length S100A8, C42S mutant, (S100 calcium-binding protein A8, Calgranulin-A, Migration inhibitory factor-related protein 8, MRP-8, Cystic fibrosis antigen, CFAG, P8, Leukocyte L1 complex light chain, Calprotectin L1L subunit, Urinary stone protein band A) cloned from human cDNA, expressed in E. coli. The protein consists of the human S100A8 (residues 1-93, UniProtKB accession P05109) with a mutation C42S .
10 20 30 40 50 MLTELEKALN SIIDVYHKYS LIKGNFHAVY RDDLKKLLET ECPQYIRKKG 60 70 80 90 ADVWFKELDI NTDGAVNFQE FLILVIKMGV AAHKKSHEES HKE
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as single band (monomer) migrating at a molecular weight below 14.4 kDa.
1 mg/mL solution in Tris 20 mM, pH 8, EDTA 1 mM, NaCl 100 mM. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 11460 M-1cm-1 calculated).
-20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.
Korndörfer, I.P., Brueckner, F. & Skerra, A. J. Mol. Biol. 370 (5), 887-898 (2007).
Ishikawa, K. et al. Acta Crystallogr. Sect. D Biol. Crystallogr. 56 (5), 559-566 (2000).
Hunter, M.J. & Chazin, W.J. J. Biol. Chem. 273 (20), 12427-12435 (1998).