MW = 23.2 kDa (dimer) calculated. Full length S100Z (S100 calcium-binding protein Z) cloned from human cDNA, expressed in E. coli. The protein consists of the human S100Z (residues 1-99, UniProtKB accession Q8WXG8).
10 20 30 40 50 MPTQLEMAMD TMIRIFHRYS GKERKRFKLS KGELKLLLQR ELTEFLSCQK 60 70 80 90 ETQLVDKIVQ DLDANKDNEV DFNEFVVMVA ALTVACNDYF VEQLKKKGK
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as single band (monomer) migrating at a molecular weight below 14.4 kDa.
1 mg/mL solution in MES 30 mM pH 6.5, NaCl 100 mM, DTT 2 mM. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280= 2980 M-1cm-1 calculated).
-20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.
Gribenko, A. V., Hopper, J.E. & Makhatadze, G.I. Biochemistry 40 (51), 15538-15548 (2001).