Calbindin D9K > Calbindin D9K - 13C15N

Bovine, recombinant
residues 5-79, P47M mutant, UniProtKB accession P02633
MW = 8.5 kDa
CAT # G02CLB01cn

This product is currently out of stock and unavailable.

SKU: G02CLB01cn Categories: ,
Catalog n.LabelingNMR StandardQtyPrice
Request a quote
Request a quote
Request a quote
Request a quote
VAT not included

For any special request or bulk quantities Click Here

Additional information

Labeling

13C15N

Qty

1 mg, 5 mg, 10 mg, 20 mg

NMR Standard

Bulk sample

Shipping in Dry Ice

no

Description

Description
MW = 8.5 kDa calculated. Calbindin D9K (Protein S100-G, CABP, Vitamin D-dependent calcium-binding protein, Intestinal) cloned from bovine cDNA, expressed in E. coli. The protein consists of Calbindin D9K, mutant P47M(residues 5-79, UniProtKB accession P02633) with 2 Ca2+ ions bound.

Sequence
        10         20         30         40         50
  M-KSPEEL KGIFEKYAAK EGDPNQLSKE ELKLLLQTEF PSLLKGPSTL 
        60         70 
DELFEELDKN GDGEVSFEEF QVLVKKISQ

Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at molecular weight below 14.4 kDa.

Supplied as
1.0 mg/mL solution in ammonium acetate 50 mM pH 6.0. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 1280 M-1cm-1 calculated).
(unless otherwise specified, NMR tubes will be sold at a concentration of 1mM)

Characteristics
Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated up to 2 mM.

Storage
-20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.
NMR USE: after the experiments return the sample at 4°C.

 
References
Kördel, J., Pearlman, D.A. & Chazin, W.J. J. Biomol. NMR 10 (3), 231-243 (1997).
Williams, R.J. Cell Calcium 20 (1), 87-93 (1996).
Skelton, N.J., Kördel, J. & Chazin, W.J. J. Mol. Biol. 249 (2), 441-462 (1995).
 
Assignments Available
1H, 13C, 15N, item 201CAB-0: J.Am.Chem.Soc. 123: 4181-4188, 2001; J.Biomol.NMR 21: 85-98, 2001
1H, 13C, 15N, item 201CAB-Ce: J.Am.Chem.Soc. 123: 4181-4188, 2001; ChemBioChem 2: 550-558, 2001
1H, 15N, items 201CAB-La, 201CAB-Nd, 201CAB-Pr, 201CAB-Sm, 201CAB-Eu, 201CAB-Tb, 201CABDy, 201CAB-Ho, 201CAB-Er, 201CAB-Tm, 201CAB-Yb: J.Am.Chem.Soc. 123: 4181-4188, 2001; Eur.J.Inorg.Chem. 2121-2127, 2002
Social media & sharing icons powered by UltimatelySocial

By continuing to use the site, you agree to the use of cookies. more information

The cookie settings on this website are set to "allow cookies" to give you the best browsing experience possible. If you continue to use this website without changing your cookie settings or you click "Accept" below then you are consenting to this.

Close