MW=16.6 kDa calculated. Full length Calmodulin with the N60D mutation, cloned from human cDNA, expressed in E. coli. The protein consists of the human Calmodulin (residues 1-148, UniProtKB accession P62158) with a N60D mutation.
10 20 30 40 50 MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ 60 70 80 90 100 DMINEVDADG DGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY 110 120 130 140 ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTA
WT – CAT # G02CLM01
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight between 14.4 and 18.4 kDa.
1.0 mg/mL solution in Tris 20 mM pH 8.0, NaCl 150 mM, CaCl2 2 mM, Complete Protease Inhibitor Cocktail EDTA-free (Roche). The concentration is calculated by the analysis of the absorbance at 280 nm (ε280= 2980 M-1cm-1 calculated). It is strongly recommended to add 1 tablet of Complete Protease Inhibitor Cocktail EDTA-free (Roche) in 1000 mL of buffer in case of buffer exchange.
-20°C. The protein is stable at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C.
Avoid repeated freeze/thaw cycles.
Bertini, I. et al. Proc. Natl. Acad. Sci. 101 (18), 6841-6846 (2004).
Ehlers, M.D. & Augustine, G.J. Nature 399 (6732), 105-108 (1999).
Kuboniwa, H. et al. Nat. Struct. Biol. 2 (9), 768-776 (1995).