MW = 7.2kDa. Recombinant α-Spectrin SH3 domain (Spectrin, non-erythroid alpha chain, Fodrin alpha chain), cloned from cDNA from chicken and expressed in E. coli. The protein consists of the soluble SH3 domain of α-Spectrin from Gallus gallus (residues 965-1025). UniProtKB accession P07751.
970 980 990 1000 M-DETGKE LVLALYDYQE KSPREVTMKK GDILTLLNST 1010 1020 NKDWWKVEVN DRQGFVPAAY VKKLD
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at molecular weight below 14.4 kDa.
1 mg/mL solution in sodium citrate 10 mM pH 3.5, NaN3 0.02% , D2O 10%. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 15470 M-1cm-1 calculated).
-20°C. The protein is stable at 4°C for months. Avoid repeated freeze/thaw cycles.
NMR USE: after the experiments return the sample at 4°C.