SH3

360,004.660,00

From chicken, recombinant
Rresidues 965-1025, UniProtKB accession number P07751
MW = 7.2 kDa
CAT # G99SH301

Add360,001.610,00
Add1.510,002.540,00
Add925,001.800,00
Add2.770,004.660,00
Add1.560,003.150,00
SKU: G99SH301-1 Categories: ,
Catalog n.LabelingNMR StandardQtyPrice
360,00
1.025,00
1.610,00
1.510,00
2.540,00
925,00
1.800,00
2.770,00
4.660,00
1.560,00
3.150,00
VAT not included

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Description

Description
MW = 7.2kDa. Recombinant α-Spectrin SH3 domain (Spectrin, non-erythroid alpha chain, Fodrin alpha chain), cloned from cDNA from chicken and expressed in E. coli. The protein consists of the soluble SH3 domain of α-Spectrin from Gallus gallus (residues 965-1025). UniProtKB accession P07751.
 
Sequence

                  970        980        990       1000
             M-DETGKE LVLALYDYQE KSPREVTMKK GDILTLLNST 
      1010       1020       
NKDWWKVEVN DRQGFVPAAY VKKLD

Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at molecular weight below 14.4 kDa.

Supplied as
1 mg/mL solution in sodium citrate 10 mM pH 3.5, NaN3 0.02% , D2O 10%. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 15470 M-1cm-1 calculated).

Storage
-20°C. The protein is stable at 4°C for months. Avoid repeated freeze/thaw cycles.
NMR USE: after the experiments return the sample at 4°C.

References
Blanco, F.J., Ortiz, Á.R. & Serrano, L. J. Biomol. NMR 9 (4), 347-357 (1997).

Additional information

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