SOD1 Cu/Zn

4.900,00

Human, recombinant
Full length, UniProtKB accession P00441
MW = 32600 Da (dimer)
EC # 1.15.1.1.
CAT # G06SD001

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Add195,004.900,00
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SKU: G06SD001-1 Category: Tags: , , ,
Catalog n.LabelingQtyPrice
170,00
520,00
840,00
2.600,00
4.400,00
195,00
600,00
970,00
3.000,00
4.900,00
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Request a quote
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Additional information

Description

Description
MW = 32.6 kDa (dimer) calculated, 32.9 kDa (metallated dimer). Human WT-SOD1 cloned from Human cDNA, expressed in E. coli. The protein consists of the full length human Cu, Zn SOD1 S-S (residues 1-154, UniProtKB accession P00441). Four additional residues, GSFT, are present at the N-term of the monomers.
 

Sequence

             10         20         30         40         50
GSFT-MATKAVCVLK GDGPVQGIIN FEQKESNGPV KVWGSIKGLT EGLHGFHVHE 
             60         70         80         90        100
     FGDNTAGCTS AGPHFNPLSR KHGGPKDEER HVGDLGNVTA DKDGVADVSI 
            110        120        130        140        150
     EDSVISLSGD HCIIGRTLVV HEKADDLGKG GNEESTKTGN AGSRLACGVI 

     GIAQ

 

Available mutant

  • Ala89Val
  • Asp90Ala *
  • Glu100Gly
  • Gly93Ala *
  • Gly93Arg
  • Gly93Asp *
  • Ile113Phe *
  • Ile113Thr *
  • Ile35Thr
  • Leu144Phe *
  • Leu148Ile
  • Leu67Val
  • Thr54Arg *
  • Val87Met *
  • Val97Met *

*Amyotrophic lateral sclerosis (ALS) related.

Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as single band (monomer) migrating at a molecular weight between 14.4 and 18.4 kDa. The Cu,Zn SOD1 S-S is stable as dimer. The metallation state is verified by NMR and/or mass analysis.
 
Supplied as
1.0 mg/mL solution in sodium phosphate buffer 50 mM, pH 7.0. The concentration is calculated by the analysis of the absorbance at 265 nm (ε265 = 15900 M-1cm-1 calculated).
 
Storage
-20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.
 
References
Banci, L. et al. PLoS One 3 (2), 1-8 (2008).
Banci, L. et al. Proc. Natl. Acad. Sci. 104 (27), 11263-11267 (2007).
Banci, L. et al. J. Biol. Chem. 281 (4), 2333-2337 (2006).
Arnesano, F. et al. J. Biol. Chem. 279 (46), 47998-48003 (2004).
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